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T & B Cell Flow Crossmatch

Test Name

T & B Cell Flow Crossmatch

Home Collection, Lab Visit

Test Code

FLOWCROSS01

Test Components/ Genes/ Parameters

Flow Crossmatch

Sample Report

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Test Information

TAT DETAILS

Next Day

PRE-TEST INSTRUCTIONS

For Recipient Collect specimen preferably 48 hours after dialysis

CLINICAL UTILITY

Cytolytic flowcytometry crossmatch is a novel method that allows for detection of even very low concenteration of preformed antibodies present in the patient serum to the donor lymphocytes

SPECIMEN STABILITY

Ship at 2-8°C, Do not freeze

METHODOLOGY

NAFlow Cytometry

STORAGE TEMPERATURE

NA

SPECIMEN TYPE

RECIPIENT: 3 mL (2 mL min,) serum from 1 SST, Collect specimen preferably 48 hours after dialysis, DONOR: 12 mL (10 mL min,) whole blood in 2 Yellow Top (ACD) tubes, RECIPIENT : 3mL serum from 1 SST

Frequently Asked Questions

T & B Cell Flow Crossmatch
The test measures very low concenteration of preformed antibodies present in the patient serum to the donor lymphocytes
NA
The test is done pretransplant to detect the antibodies in recipient serum against the donor
Flow Cytometry
No risk
A positive flowcross match suggests increased risk of allograft rejection. A negative cross match does not imply that the donor and the patient are related.